ISHS Acta Horticulturae 331: WOCMAP I - Medicinal and Aromatic Plants Conference: part 3 of 4
INACTIVATION OF XANTHOMONAS CAMPESTRIS PVS. IN VITRO WITH PLANT EXTRACTS |
| Authors: | A.A. Csizinszky, J.B. Jones, E.L. Civerolo |
| Keywords: | Bacterial leaf spot, Hungarian chamomile, Matricaria recutita L., Roman chamomile, Chamaemelum nobile (L.) All |
| Abstract:
The effects of Hungarian chamomile, [Matricaria recutita (L.) Rausch], and Roman chamomile [Chamaemelum nobile (L.) All], extracts on Xanthomonas pvs. were determined in vitro. Three strains (XC 62, Japan; XC 64, Argentin; and XC 70, Brasil) of the causative agent of the citrus bacterial canker disease Xanthomonas campestris pv. citri and a strain of X.c. pv citri associated with citrus bacterial spot in Florida, were each incubated with three concentrations of chamomile extracts. Chamomile extracts at concentrations of 0.5, 0.75, and 1.0 ml L-1 were diluted in 1% EtOH containing 0.08% Tween 20. Bacterial suspensions containing 1 x 106 cfu ml-1 were prepared in 0.01 M, pH 7, phosphate buffer (PBS), pipetted into the chamomile extracts and incubated for 30 min. Ten μl of the treated suspensions were transferred onto 0.2% nutrient glucose agar (NGA) plates in triplicate. Controls were treated similarly. Hungarian chamomile extract inhibited the growth of the Asian, Argentinian, and Floridian, but not the Brasilian strain at all three concentrations. Roman chamomile extract at 0.75 and 1.0 ml L-1 inhibited the growth of all four strains, but slight growth of strain XC 70 (Brasil) occurred at the 0.5 ml L-1 concentrations. | |
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