ISHS Acta Horticulturae 330: WOCMAP I - Medicinal and Aromatic Plants Conference: part 4 of 4
RAUWOLFIA CULTURED CELLS: INDOLE ALKALOIDS PRODUCTION AND THEIR DETERMINATION |
| Authors: | S.I. Gubar, E.P. Konstantinova, V.A. Kunakh |
| Keywords: | Microchromatography, Ajmalicine, Ajmaline, Reserpine |
| Abstract:
Quantitative method for estimation of indole alkaloids were elaborated by microcolumn chromatograph. The developed method permits carrying out simultaneous determination of ajmalicine, reserpine, vomilenine, ajmaline and serpentine in the tussue culture extracts. Various Rauwolfia cell lines have been analysed. About 30 different alkaloids were detected and contents of some alkaloids were measured in the cultured cells including alkaloids used in medicine (ajmalicine, reserpine, ajmaline, serpentine). | |
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