ISHS Acta Horticulturae 329: VII International Symposium on Plant Growth Regulators in Fruit Production
THE ROLE OF ENDO-1,4-BETA-GLUCANASE IN PLANT CELL ELONGATION. IN-VITRO STUDIES OF PEACH POLLEN |
| Authors: | O. Shoseyov, M. Dekel-Reichenbach |
| Keywords: | Prunus persica, cv. Texas, cellulase, CMCase |
| Abstract:
A bacterial endo-1,4-beta-glucanase ('cellulase'), homologous to plant 'cellulase', was cloned from Clostridium cellulovorans. The gene (engC) was subcloned into E. coli and was found to express an active Carboxy Methyl Cellulase (CMCase) enzyme (Shoseyov et al 1990). Recently it was proposed that endo-1,4-beta-glucanase plays a major role in plant cell elongation. We developed a plant cell elongation model system in peach pollen, in order to test in vitro the effect of recombinant endo-1,4-beta- glucanase on peach pollen tube elongation. It was found that recombinant EngC promotes pollen tube elongation more then twice as the control, thus supporting the hypothesis that engC belongs to a unique type of genes that represents convergent evolution, and any homologous sequences may reflect important functional domains. | |
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