ISHS Acta Horticulturae 329: VII International Symposium on Plant Growth Regulators in Fruit Production
UPTAKE AND METABOLISM OF INDOLE-3-BUTYRIC ACID AND INDOLE-3-ACETIC ACID BY PETUNIA CELL SUSPENSION CULTURE |
| Authors: | E. Epstein, O. Sagee, A. Zelcer |
| Keywords: | Autofluorography, IBA glucose, IBA aspartic acid |
| Abstract:
The uptake and metabolism of indole-3-acetic acid (IAA) and indole-3-butyric acid (IBA) were studied in suspension cell cultures of Petunia hybrida. The uptake of IAA and IBA seemed to follow different courses, probably by different mechanisms. IBA was metabolized very rapidly by the cell suspension to two new compounds which were identified tentatively as IBA aspartic acid (IBAasp) and IBA glucose (IBAglu), but after 24 h only IBAasp was present. Most of the IBA in the medium was converted in 24h to a new metabolite (probably IBAasp). IAA metabolism proceeded at a slower rate and was also converted rapidly to two new metabolites. We postulate that Petunia cells conjugate IBA rapidly to IBAglu which in turn is converted to form IBAasp which probably acts as a "slow release" hormone. | |
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