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ISHS Acta Horticulturae 325: VI International Symposium on Flower Bulbs

PROPAGATION OF SHOOTS AND BULB GROWTH OF TULIP IN VITRO

Authors:   M. Hulscher, H.T. Krijgsheld, P.C.G. van der Linde
Abstract:
A method for micropropagation of tulip is described. Shoots were generated from stem explants and axillary buds on media with a-naphthaleneacetic acid (NAA), N6-[2-isopentenyl]adenine (2iP) and 6-benzylaminopurine (BAP) or Zeatine. Shoots with a meristem were selected. Propagation of these shoots was possible by cutting them longitudinally. Single shoots formed bulbs after a cold treatment on a medium with 70 g/l sucrose without plant growth regulators. These bulbs were planted in soil. They emerged for 30–100% depending on the weight of the bulbs. After the growing season new bulbs were harvested from almost every sprouted bulb.

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