ISHS Acta Horticulturae 319: International Symposium on Transplant Production Systems
INFLUENCE OF DIFFERENT TEMPERATURE TREATMENTS ON VIABILITY OF IN VITRO CULTIVATED ALLIUM SHOOTS AND BULBLETS |
| Author: | J. Keller |
| Keywords: | Ion leakage, growth characteristics, germplasm preservation |
| Abstract:
In the genebank Gatersleben, one of largest European living plant collections, efforts are made to establish an in vitro storage unit for vegetatively propagating Allium accessions. Investigations on cold as well as on heat influences are necessary to improve the storage conditions and to contribute to thermotherapy for pathogen elimination measures. Five parameters were used for viability estimations in various Allium material (garlic, onion, shallot, top onion, and rakkyo): differences in fresh weight, length difference, root formation, total ion as well as K+ ion leakage. The measures could be demonstrated to be sufficiently distinctive, but the significances in leakage data are lower than in the morphogenetic characters. In cold treatments at -15, -2, +2 +10 °C, storage at -2 °C is as favourable as at +2 °C, differences in cold hardiness were recorded within different plant material. Heat treatments at 45 °C led to higher damages when combined with illuminaton. Stepwise increase of temperatures caused adaptation effects in plants. | |
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