ISHS Acta Horticulturae 316: International Workshop on Intensive Cultivation of Protea
MICROPROPAGATION OF SELECTED LEUCOSPERMUM CORDIFOLIUM: EFFECT OF ANTIBIOTICS AND GA3 |
| Authors: | E. Tal, H. Solomon, J. Ben-Jaacov, A.A. Watad |
| Abstract:
The establishment of a dwarf clone of Leucospermum cordifolium in culture was difficult because of heavy endogenous bacterial contamination. Two-nodal segments were placed on half strength Murashige and Skoog medium supplemented with 6-benzyl-aminopurine to which the antibiotics streptomycin and rifampicin were added. The addition of 100 mg.l-1 rifampicin to the growth medium was most effective in suppressing the bacteria and enabling the growth of the Leucospermum. GA3 at 1–2 mg.l-1 was found to be essential for rapid shoot proliferation and elongation. Shoots multiplied at the rate of approximately five new shoots within 8 weeks. The best rooting was achieved when 1 mg.l-1 indole butyric acid (IBA) was added to the medium. Relatively high light intensities promoted in vitro rooting. Following rooting, plants were successfully transferred to outdoor conditions. | |
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