ISHS Acta Horticulturae 316: International Workshop on Intensive Cultivation of Protea
PROPAGATION OF PHAENOCOMA PROLIFERA IN IN VITRO CULTURE |
| Authors: | G. Kaddar, A.A. Watad, T. Ben-Noon, J. Ben-Jaacov |
| Abstract:
Phaenocoma prolifera of the Asteraceae family is an evergreen shrub, widely distributed along the coast of the south western Cape of South Africa. A micropropagation method was developed in order to propagate special plant forms with high potential as flowering pot plants. The addition of growth regulators to Murashige and Skoog's medium at the initial stage in culture has a negative effect on shoot development, and much callus developed. However, after a few subcultures, addition of 1 mg.l-1 6-benzyl-aminopurine (BAP) promoted shoot growth and proliferation. Rooting of the in vitro mini-cuttings was achieved by 1 mg.l-1 1-naphthalene acetic acid (NAA). | |
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