ISHS Acta Horticulturae 316: International Workshop on Intensive Cultivation of Protea
IN VITRO ESTABLISHMENT OF PROTEA OBTUSIFOLIA |
| Authors: | A.A. Watad, J. Ben-Jaacov, S. Cohen, E. Tal, H. Solomon |
| Abstract:
In vitro establishment of Protea obtusifolia faces four major problems: browning of tissue and media, no sprouting of axillary buds, endogenous contamination, and tissue vitrification. Multinodal shoots segments of P. obtusifolia were established. Etiolation treatment of the new actively growing shoots on the mother plants was helpful to overcome browing and promoted sprouting of axillary buds. Addition of gibberellic acid (GA3) to the basal medium supplemented with 1 mg.l-1 benzylaminopurine (BAP) promoted bud sprouting and elongation. | |
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