ISHS Acta Horticulturae 314: II International Symposium on Propagation of Ornamental Plants
MICROPROPAGATION OF PAEONIA |
| Authors: | M.R.J. Albers, B.P.A.M. Kunneman |
| DOI: | 10.17660/ActaHortic.1992.314.9 |
| Abstract:
We developed a micropropagation protocol for Paeonia. Surface sterilized explants with one node were placed on a medium based on Lepoivre. The contamination percentage varied between 22–100 for the herbaceous peonies, but was negligible for the tree peonies. The multiplication rate was 1.3 to 2.9 in ca 7 weeks, at 15 °C, a light intensity of 35 mmol/s/m2 and a daylength of 16 hrs. We examined several factors in order to improve the multiplication rate None of these factors appeared to increase the multiplication rate significantly. The only significant effect on the length of the shoots was a cold treatment. Rooting was better with IAA or IBA than with NAA, the optimal concentration being about 0.1 mg/l. Rooting in the dark was better than in white or red light. About 50% of the plantlets was lost due to infection during weaning. Non-infected plantlets grew well. The first plants have just been planted outside, after one growing season in the greenhouse. | |
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