ISHS Acta Horticulturae 314: II International Symposium on Propagation of Ornamental Plants
IN VITRO REGENERATION OF CAMELLIA HYBR. CV. DEBBIE FROM LEAF AND STEM EXPLANTS |
| Authors: | A. Tosca, R. Pandolfi, A. Macchi |
| Keywords: | BA, cefotaxime, IBA, thidiazuron, TIBA |
| Abstract:
In woody ornamental species conventional breeding is a time consuming process. Through in vitro culture, useful mutant somaclones can be achieved. In order to obtain such somaclones in Camellia hybr. cv. Debbie a method to regenerate whole plants in vitro is needed. Light and dark conditions, source of explants from either in vitro or in vivo plants and medium composition were assessed. Photoperiodic conditions have been proved to be a key factor for the regeneration of shoots. In our experiments internodal stem segments performed better than leaf explants. Stems taken from in vivo plants produced as many regenerating calli as those from in vitro. IBA showed to be necessary with stems, but not with leaves. TDZ at the doses tested did not overcome BA regeneration rate. The highest TIBA dose (1 mg/l) enhanced organogenesis; cefotaxime (50–100 mg/l) highly improved regeneration of explants from in vivo plants. | |
Download Adobe Acrobat Reader (free software to read PDF files) | |
