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ISHS Acta Horticulturae 314: II International Symposium on Propagation of Ornamental Plants

GENETIC VARIATION DETECTED BY DNA FINGERPRINTING IN FLOWERS

Authors:   A. Vainstein, J. Hillel, U. Lavi, G. Tzuri
Keywords:   breeding, carnation, cultivar identification, gerbera, relatedness, rose
Abstract:
Minisatellite DNA probes hybridize to many loci in rose, gerbera and carnation DNA, thus enabling cultivar identification in these ornamentals. The level of polymorphism revealed by DNA fingerprints is dependent upon the probe/genus combination. Genetic relatedness between carnation genotypes belonging to phenotypical categories 'standard', 'spray' and 'dwarf' was studied by DNA fingerprint analysis. A comparison of genetic relationships within and between categories based on known genetic history, to genetic relationships deduced from DNA fingerprints revealed a perfect match. It is apparent from our results that the application of DNA fingerprinting methodology to the plant kingdom can greatly assist in cultivar identification, breeding programs and evolutionary research.

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