ISHS Acta Horticulturae 314: II International Symposium on Propagation of Ornamental Plants
EVIDENCE FOR BUD ORIGIN INFLUENCING PERFORMANCE DURING MICROPROPAGATION |
| Authors: | T. R. Marks, P. E. Myers |
| Keywords: | Betula pendula, Daphne odora, explant, in vitro |
| Abstract:
The selection of explants from trees and shrubs at culture initiation is often restricted to the apical buds of vigorously growing shoots. Apparently dormant axillary buds lower down the shoot, as well as weaker shoots on the plant, are often ignored. Bud growth and further development in vitro, however, are dictated by bud topophysis, and in some cases by the potential growth habit of shoots on the stockplant. In those where shoot growth is achieved only by apical extension (e.g. Daphne), the maximum growth potential in vitro lies within the apex or adjacent axillary buds. In plant species where annual shoots produce lateral branches (e.g. Betula), maximum growth potential in vitro is found in buds which would normally develop into lateral shoots. In non-branching shoots from the same Betula plant the pattern of bud performance is more similar to that of Daphne shoots. Explant origin also affects the rooting of culture-lines, and can be traced in Betula ten months after initiation. Buds on shoots growing in vitro also express differential growth potential paralleling that of the original explant selection. In Daphne this not only affects shoot multiplication and rooting, but also the branching habit of the micropropagated plant ex vitro. | |
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