ISHS Acta Horticulturae 314: II International Symposium on Propagation of Ornamental Plants
EFFECTS OF TISSUE CULTURE AND ACCLIMATIZATION CONDITIONS ON THE SURVIVAL AND GROWTH OF ROOTED AND UNROOTED MALUS AND PYRUS MICROCUTTINGS |
| Authors: | B.P.A.M. Kunneman, M.R.J. Albers |
| Abstract:
Micropropagated plants often die as a result of suboptimal conditions during acclimatization, rooting and multiplication. We investigated the effects of saccharose concentration in the multiplication phase on in vitro rooting and acclimatization of Malus. An increase of saccharose concentration from 20 to 30–35 g.l-1 during multiplication improved the multiplication rate, rooting and percentage of survival. We also examined the effects of temperature and peat-perlite mixtures with 35, 50, 70 and 90 % peat on Pyrus during acclimatization. Rooted microcuttings survived better than unrooted cuttings. Temperature had no obvious effect on survival and growth. The percentage of the plants that survived was higher in peat-perlite mixtures with 35 % peat than in mixtures with 50, 70 or 90 % peat. Promotion of growth however, occurred in mixtures with a higher percentage of peat. The opposite effect of peat-perlite mixtures on rooting, survival and growth might be explained by the difference in fertilization. There was no effect of IBA pre-treatment (25, 50, 100 and 200 mg.l-1 on in vivo rooting of microcuttings. | |
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