ISHS Acta Horticulturae 314: II International Symposium on Propagation of Ornamental Plants
ON THE RELATION BETWEEN VITRIFICATION AND STOMATAL CELL WALL DEFORMITY IN CARNATION LEAVES IN VITRO |
| Authors: | M. Ziv, T. Ariel |
| Abstract:
Hyperhydrous shoot development - vitrification - affects the survival and quality of several micropropagated plants ex vitro. The leaves which are the immediate organs to be affected, exhibit abnormal morphology and physiology. The medium's physical state and the level of NH4+ in the medium affected leaf development. Vitreous leaves evolved higher levels of ethylene, contained callose and were lower in proteins. The failure of stomata in vitreous leaves to close resulted from structural changes in their cell walls as revealed by various microscopic studies. The correlation found between stomatal malformation and vitrification is discussed in an attempt to evaluate leaf hyperhydration more accurately. | |
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