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ISHS Acta Horticulturae 311: International Walnut Meeting

MICROPROPAGATION OF HYBRID WALNUT TREES: SOME FACTORS INVOLVED IN ROOTING

Authors:   C. Jay-Allemand, S. Peng, P. Capelli, D. Cornu
Abstract:
Several factors controlling in vitro rooting of interspecific hybrid walnut shoots have been studied. The rooting rates and the root number per rooted shoot were markely increased when shoots had been disbudded and myricetin (a flavonol) added in the culture medium, during the induction phase. Furthermore, roots were induced, without auxin, by serotonine (an aromatic polyamine) associated to juglone (a naphtoquinone). Endogeneous content of flavonols (quercitrin and myricitrin) and naphtoquinones (hydrojuglone glucoside and juglone) were quantified in shoots by means of HPLC throughout induction phase and correlated with root formation. Finally, in vitro root development was strongly stimulated by a new substrate for walnut which is a mixture of DKW gelified medium and vermiculite used in good proportions. This procedure resulted in 80 to 100% of rooting for 5 hybrid clones and produced a better quality of rooted plants for acclimation.
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