ISHS Acta Horticulturae 309: XV International Symposium on Fruit Tree Diseases
EVIDENCE FOR THE SPREAD OF PRUNUS NECROTIC RINGSPOT VIRUS AND THE PRESENCE OF PRUNE DWARF VIRUS IN SELECTED PEACH ORCHARDS IN SOUTH CAROLINA |
| Authors: | S.W. Scott, R. Walker Miller, E. J. Bachman |
| Abstract:
Orchards of two peach cultivars, Coronet and Redglobe, visited in 1987 during a survey to determine the prevalence of Prunus necrotic ringspot virus (PNRSV) in the state of S. Carolina, were revisited in 1991. Samples were collected using the procedures adopted in 1987 (Scott, et al., 1989) and were assayed for the presence of PNRSV and prune dwarf virus (PDV) by ELISA. For some orchards the samples were also assayed for the presence of PDV using a nucleic acid probe. Substantial increases in the incidence of PNRSV were noted. PDV was detected both by ELISA and by using a nucleic acid probe. Agreement between the results obtained for the two methods was good. However, the sensitivity of the nucleic acid probe made discriminating between PDV-infected and healthy trees much easier than when using ELISA. Although trees infected with both PDV and PNRSV were detected, symptoms of peach stunt disease were not observed. | |
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