ISHS Acta Horticulturae 309: XV International Symposium on Fruit Tree Diseases
ENZYMATIC CDNA AMPLIFICATION OF HOP STUNT VIROID VARIANTS FROM NATURALLY INFECTED FRUIT CROPS |
| Authors: | A. Hadidi, Y. Terai, C.A. Powell, S.W. Scott, J.C. Desvignes, L.M. Ibrahim, L. Levy |
| Keywords: | Detection, Polymerase chain reaction (PCR), Prunus, Viroids |
| Abstract:
Hop stunt viroid (HSV) cDNA reverse transcribed in vitro from total nucleic acid extracts of infected tissue was amplified using Taq I DNA polymerase and HSV group-specific DNA primers in a reverse transcription-polymerase chain reaction (RT-PCR). The RT-PCR assay is a rapid and specific method to determine HSV infection in fruit crops without viroid purification or the need for large amounts of infected tissue. | |
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