ISHS Acta Horticulturae 309: XV International Symposium on Fruit Tree Diseases
PARTIAL CHARACTERIZATION OF A CLOSTEROVIRUS-LIKE PARTICLE TRANSMITTED FROM CHERRY (PRUNUS AVIUM) INFECTED WITH SEVERAL VIRAL DISEASES |
| Author: | D. JAMES |
| Abstract:
A high pH buffer containing magnesium, sodium thioglycolate, and nicotine was used successfully to transmit a closterovirus-like particle from sweet cherry (Prunus avium) to the herbaceous host Chenopodium quinoa. Chlorotic spots and stunting were observed on C. quinoa plants 17 days post inoculation. As a result of host range studies C. amaranticolor was identified as a local lesion host of the virus. Analysis of the virus coat protein by SDS-polyacrylamide gel electrophoresis revealed the presence of a single protein species with a molecular weight of 20,500 daltons. | |
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