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ISHS Acta Horticulturae 309: XV International Symposium on Fruit Tree Diseases

EVALUATION OF RESISTANCE TO PLUM POX VIRUS IN APRICOT TREES

Authors:   F. Dosba, S. Orliac, F. Dutrannoy, P. Maison, G. Massonié, J.M. Audergon
Keywords:   Plum pox virus, aphid transmission, chip-budding, in vitro culture
Abstract:
Since 1985, a study has been carried out to investigate thebehaviour of several varieties or hybrids of apricot to Plum pox virus (PPV). This involved the examination of 88 apricot varieties or clones originating from different parts of the world, 171 hybrids originating more specifically from the cross [Screara x Stark Earli Orange], and three progenies with Stark Earli Orange or Henderson as genitors. Plants remained ungrafted or were grafted onto PPV susceptible rootstocks of peach (GF305 or Rubira) and inoculated with PPV using either the chip-budding or aphid transmission methods. Two strains of PPV were used for the inoculations: the D strain originating from infected apricots in the south-eastern part of France, and the Marcus (M) strain isolated from peach in Greece. Inoculations were also developed on young plantlets transferred to the greenhouse following in vitro culture.

Symptoms of PPV infection developed more rapidly following inoculation by aphid transmission than by chip-budding, and the response of the plants to the M strain was generally greater than for the D strain. Some apricot varieties showed a very high level of PPV resistance, occuring mainly in varieties originating from North America, i.e., Veecot, Stella, Stark Earli Orange et Henderson. A significant level of resistance was transmitted from Stark Earli Orange to the progeny since 29% of the hybrids [Screara x Stark Earli Orange] appeared resistant.

Preliminary results concerning the evaluation of PPV resistance following in vitro culture, and the different approaches adopted to introduce this resistance into apricot varieties in France, are presented.

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