ISHS Acta Horticulturae 309: XV International Symposium on Fruit Tree Diseases
IN VITRO AND IN VIVO EXPRESSION OF PPV HELPER-COMPONENT GENE |
| Authors: | M. Ravelonandro, G. Tavert-Roudet, L. Garrigue, O. Peyruchaud, J. Dunez |
| Abstract:
In vitro translation experiments have been carried out with cRNAs obtained by transcription of cloned cDNAs representing the first two cistrons and the N-terminal part of the third cistron of plum pox virus (strain D): our results show that the polypeptide encoded by this genomic segment has proteolytic activity. The protease activity was located to the C-terminal region of the polyprotein precursor corresponding to plum pox virus cistrons 1 and 2, like the helper-protease of the tobacco etch and tobacco vein mottling potyviruses. | |
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