ISHS Acta Horticulturae 308: XV International Symposium on Small Fruit Virus Diseases
ARABIS MOSAIC VIRUS: DETECTION BY SP6-GENERATED CRNA PROBES AND POSSIBLE RECOMBINATION WITH STRAWBERRY LATENT RINGSPOT VIRUS |
| Authors: | A. Hadidi, P. Piazzolla, V. Savino, M.F. Clark |
| Abstract:
A cDNA clone was constructed for RNA-2 of arabis mosaic virus (ArMV) and then subcloned into the SP6 transcription vector pSP64. High specific activity 32P-labelled ArMV cRNA transcripts were generated and used as hybridization probes to detect the ArMV genome and related sequences. In Northern blot hybridization experiments, ArMV cRNA hybridized to ArMV RNA-2 from purified virions of the hop isolate as well as nucleic acid extracts of tissue infected with the hop, strawberry, blackberry, grapevine, or ash isolate. The probes did not hybridize to nucleic acids isolated from uninfected tissue or to RNA isolated from purified tobacco ringspot virus, tomato ringspot virus, tomato bushy stunt virus, cucumber mosaic virus, or tobacco mosaic virus. Strawberry latent ringspot virus (SLRV) RNA-1, but not SLRV RNA-2 hybridized to the probes. These results indicate that our ArMV cRNA probes can be used for the detection of the above isolates of ArMV. Further, it suggests possible recombination between ArMV RNA-2 and SLRV RNA-1 in nature. | |
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