Abstract:
In these studies presented here, W. somnifera plants originated from two different sources and ecological regions are cultivated in Cukurova region-Turkey.
During the two years of field trials, in order to test ontogenetic variability, three different harvests were applied, representing early-reproductive, reproductive and post-reproductive development stages.
The results showed that the highest drug herb and drug folis yield were obtained from the harvest at the post-reproductive stage, and that the drug yields of two ecotypes used as experimental material were significantly different from each other.
Since the Withanolides are highly oxygenated steroids and differ principally in the positions of the hydroxyl groups and in the locations of the double bonds, they display a wide range of polarity which gives good separation by HPLC. The determination of the main withanolide of the samples of W. somnifera cultivated at the experimental farm of Cukurova University was carried out using reversed-phase HPLC. According to the results of the analysis, plants originated from Izmir and Adana were found to have different chemotypes.
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