ISHS Acta Horticulturae 306: International Symposium on Medicinal and Aromatic Plants, XXIII IHC
INTRODUCTORY STUDY FOR THE OPTIMIZATION OF GROWTH AND ALKALOID PRODUCTION BY CELL CULTURES OF DATURA STRAMONIUM L.. |
| Authors: | K. Demeyer, F. Vanhaste, H. Van de Velde, R. Dejaegere |
| Abstract:
Calli were induced from leaves, stems and roots of the five D. stramonium varities (chalybea, godronii, inermis, stramonium, tatula), and cultivated in the dark as well as under a 16 h light/8 h dark light cycle. Hereby, calli were grown on MS media characterized by different 2, 4 D and BAP concentrations and ratio's. For all varieties higher biomass yields were observed when calli were cultured under 16 h light/8 h dark, whereas highest yields were found with the variety tatula. As for the influence of both growth regulators, good results were generally obtained with the medium containing 0.1 mg/l 2,4 D + 0.5 mg/l BAP for calli grown in the light, except for the variety tatula. Here, higher yields were found with a medium containing 0.5 mg/l 2,4 D + 0.1 mg/l BAP. This latter medium also showed good results with calli grown in the dark, and this for the five varieties. The varieties chalybea, inermis and stramonium were further analysed for their alkaloid content (hyoscyamine + scopolamine). Nearly always, a higher content was found in calli grown in the dark. Also for the influence of 2, 4 D and BAP an inversed relationship was found between alkaloid content and biomass yield. These results point into the direction of an indirect effect, meaning that the parameters investigated influence alkaloid content by their effect on biomass production. However, also a direct influence of 2,4 D and BAP was noticed. With the medium containing the lowest concentrations of both growth regulators (0.1 mg/l 2,4 D + 0.1 mg/l BAP) an intermediate biomass yield was accompanied by a well marked higher alkaloid content. | |
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