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ISHS Acta Horticulturae 300: In Vitro Culture, XXIII IHC

CYTOLOGYCAL CHARACTERIZATION OF CELL SUSPENSION CULTURES OF FRUIT TREES

Authors:   F. Blando, L. Giorgetti, M.G. Tonelli, V. Nuti Ronchi
Abstract:
Using a new suitable method to initiate a cell suspension culture in apple (Malus x domestica Borkh.) and quince (Cydonia oblonga Mill.), it has been possible to detect some cytological mechanisms of chromosome reduction, firstly reported in carrot cell cultures (Nuti Ronchi, 1990; Nuti Ronchi et al., 1990). These segregational events, namely somatic-meiosis and prophase-reduction, are present in all analysed cultures, haploid metaphases (n=17) being 3.2% in apple lines. The importance and role of these phenomena in cell cultures of different non-embryogenic species are discussed in comparison with the carrot cell culture model.

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