ISHS Acta Horticulturae 300: In Vitro Culture, XXIII IHC
OVULE CULTURE OF SEEDLESS GRAPES (VITIS VINIFERA L.) CV. PERLETTE |
| Authors: | Z. Singh, S.J.S. Brar, S.S. Gosal |
| Abstract:
In-ovulo embryo culture of Vitis vinifera L. cv. Perlette was investigated as a method to enable the hybridization of stenospermic seedless grapes. Fertilized ovules 10–40 days after anthesis were cultured on three basal media viz. (1) MS (1962) medium, (2) White's (1954) medium and (3) Nitsch's (1969) medium supplemented with various combinations and concentrations of 2,4-D, NAA, IAA, Kinetin, BAP, gibberellin A4/A7, L-cysteine and L-asparagine. Percent survival of cultured ovules was maximum on MS fortified with IAA (2 mg/l) and BAP (0.5 mg/l). In general, physical status viz. filter paper bridges put in liquid medium and agar solidified cultured media did not affect per cent survival much. The cultured ovules resumed growth within a week and remained green throughout its development in vitro. The addition of L-asparagine (10 mM) or L-cysteine (10 mM) to White's medium or Nitsch's medium promoted growth of the ovules. Most of the ovules were hollow and contained no visible embryo or endosperm remnants even when outer integument tissue remained green. The callus was produced by outer layer of the outer integument. | |
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