ISHS Acta Horticulturae 300: In Vitro Culture, XXIII IHC
STUDY OF THE EFFICIENCY OF GARDENIA SHOOT FORMATION IN VITRO |
| Authors: | J.G. Berrios, A.S. Economou |
| Abstract:
Gardenia explants, from in vitro-derived shoots, of various types and sizes and different handling during preparation and establishment were tested for their efficiency on shoot formation and growth. Explants of shoot apex and nodal segment produced more shoots with more internodes than shoot tip and decapitated shoot tip explants. Shoot apex explants with a size of 0.5 cm and less were more prolific than longer explants. In nodal segment explants, the various sizes tested did not differ from each other in shoot formation. Horizontal or vertical insertion of the explant into the medium did not alter shoot production and growth; however, leaf removal from shoot apex explants caused a reduction in shoot production. | |
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