ISHS Acta Horticulturae 300: In Vitro Culture, XXIII IHC
NON-HORMONAL FACTORS THAT IMPROVE THE MULTIPLICATION AND DEVELOPMENT OF SOMATIC EMBRYOS OF CUCUMBER (CUCUMIS SATIVUS, L.). |
| Authors: | J. A.R Ladyman, B. Girard |
| Abstract:
This report describes the effect of selected non-hormonal factors on the development of somatic embryos of cucumber, (Cucumis sativus L.) cv. "Clinton". Somatic embryos were induced on cotyledonary explants. Multiplication, and subsequent conversion of somatic embryos, was influenced by gelling agent, carbohydrate source, transfer frequency and photoperiod. The presence of activated charcoal led to premature germination of the somatic embryos. Tissue was transferred, at least, once every four weeks. The greatest number of regenerants were produced when embryogenic tissue was multiplied on media gelled with Gelrite (0.15%), with sucrose (3%) as the carbon source and incubated under a 12-hour photoperiod (37 uE/m2/s). In this case, 50 mg of putative embryogenic tissue yielded 56 vigorous seedlings. | |
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