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ISHS Acta Horticulturae 300: In Vitro Culture, XXIII IHC
ENDOGENOUS POLYAMINE AND ROOT MORPHOGENESIS VARIATIONS UNDER DIFFERENT TREATMENTS IN CUTTINGS AND IN IN VITRO EXPLANTS OF OLIVE
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| Authors: | E. Rugini, M. Luppino, S. Grego, M. de Agazio |
Abstract:
Rooting of in vitro explants and in vitro cuttings of olive tree (Olea europaea L.) were followed after different basal treatments with putrescine, H2O2, and mannitol with or without IBA. The results showed a general increase of rooting and an earlier root formation when all substances were combined with IBA treatment.
The analysis of polyamine content (putrescine, spermidine and spermine) at 1, 3 and 7 days from the beginning of the treatments, revealed an exceptionally high content of conjugated spermidine at the 3rd day, both in 2 cm basal cuttings and in leaves, with putrescine and H2O2 treatment.
We discuss a possible role of spermidine during root induction and the possible link among the high spermidine content, its degradation products and the known physiology of olive cuttings.
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