ISHS Acta Horticulturae 300: In Vitro Culture, XXIII IHC
IN VITRO MANIPULATIONS OF PYRUS SPECIES AND CYDONIA OBLONGA |
| Authors: | R. Dolcet-Sanjuan, D.W.S. Mok, M.C. Mok |
| Abstract:
This paper presents highlights of research centered on in vitro manipulations of pear and quince. The results describe: 1) optimization of micropropagation procedures; 2) induction of adventitious shoot formation; and 3) characterization and selection of rootstocks with differential responses to low Fe availability. A particularly important condition for micropropagation was high light intensity (135 μmolm-2s-1) which enhanced the multiplication rate and enlarged the leaf size in Pyrus. Adventitious shoots were regenerated from leaf discs of P. amygdaliformis Vill. and Cydonia oblonga L. on medium containing thidiazuron (TDZ) and  -naphthaleneacetic acid (NAA). The optimal combination was 3 μM TDZ and 1 μM NAA for P. amygdaliformis, and 32 μM TDZ and 0.3μM NAA for C. oblonga. Micropropagated shoots, plantlets, and roots of the two species differed dramatically in the ability to reduce Fe(III). Roots of P. amygdaliformis, a species with high tolerance to low Fe availability, reduced Fe(III) at a rate 12 times higher than C. oblonga, which is sensitive to low Fe.
The adventitious shoots obtained from C. oblonga leaf discs have been used to screen for somaclonal variants with elevated tolerance to low Fe.
| |
Download Adobe Acrobat Reader (free software to read PDF files) | |
