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ISHS Acta Horticulturae 300: In Vitro Culture, XXIII IHC

EFFECT OF LIGHT ON ROOTING IN VITRO OF PETUNIA MICROSHOOTS

Authors:   C. Cabaleiro, A.S. Economou
Abstract:
Photoperiod of 24 h enhanced root formation in petunia microshoots 5 days after incubation in MS hormone-free medium. However, there were no differences in rooting on the 10th or the 15th day after incubation between 24 h and 14 h photoperiods. Microshoot rooting increased linearly from an irradiance of 20 μmol.s-1.m-2 to 60 μmol.s-1.m-2. The number of roots produced, their length and weight, also increased from the lowest to the highest irradiance used. Charcoal in the medium arrested rooting, whereas covering the upper part of the test tube surrounding the microshoot with black polyethylene had no negative effect on rooting.

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