ISHS Acta Horticulturae 300: In Vitro Culture, XXIII IHC
IN VITRO ROOTING OF PYRUS CALLERYANA |
| Authors: | G. Berardi, D. Neri, A. Maiorino, R. Adversi |
| Abstract:
Five Pyrus calleryana genotypes were tested in order to determine an effective method to induce root formation in vitro. Micropropagated shoots originated from shoot tip explants (two selected ornamental clones, N and CH, and the rootstock D6) and from in vitro embryo culture (clones 11 and 13) were used. The effect of two auxins (IBA and NAA), phloroglucinol (PG) and initial darkness treatments on root induction were studied. Early transplanting was carried out to reduce in vitro culture time after root initiation. Shoots showed better rooting without auxins and with PG (162 mg1-1) in clones 11 (44%) and 13 (26%). Clones N and D6 needed an initial darkness treatment of at least 3 days and the presence of NAA (0.5–1 mg1-1) to increase rooting, reaching 75% in clone D6. Root emergence in clone CH was reduced by callus proliferation. Histological studies revealed root induction had already occured after 3 days on rooting medium in the dark conditions for all the clones. Transplanting was successful only after 21 days of in vitro culture. | |
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