ISHS Acta Horticulturae 300: In Vitro Culture, XXIII IHC
IN VITRO PROPAGATION OF APPLE: COMPARATIVE RESPONSE OF THREE CULTIVARS TO CYTOKININ AND AUXIN |
| Authors: | A.M. ABDUL KADER, A. MATHÉ, K. LASZLOFFY |
| Abstract:
Successful in vitro shoot proliferation of three apple cultivars Malus domestica Borkh. 'Granny Smith', 'Mutsu', and 'Spur Golden Delicious' was accomplished. Vegetative axillary buds obtained from field trees were surface-sterilized and incubated on MS (1962) nutrient medium with half-strength macro nutrients containing 0.5, 0.7, and 1.0 mg BA/l, and 0.1 mg IBA/l. The optimal BA concentrations for shoot proliferation were 0.7 mg/l for Mutsu, 1.0 mg/l for ''Granny Smith' and 0.5 mg/l for 'Spur Golden Delicious'. At these concentrations the average number and length of shoots was as follows: 'Granny Smith' 7.5/2.7 cm, 'Mutsu'' 10.4/3.6 cm and 'Spur Golden Delicious' 7.7/4.9 cm. Root induction was accomplished by using agar gelled and liquid media with different concentrations of auxins (IBA, NAA, and IAA). Survival rates after transplanting were satisfactory. | |
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