ISHS Acta Horticulturae 300: In Vitro Culture, XXIII IHC
IN VITRO PROPAGATION OF 'JULYRED' APPLE |
| Authors: | K. LASZLoFFY, A.M. ABDUL KADER, A. MATHÉ |
| Abstract:
Tissue culture propagation of Malus domestica Borkh cv. Julyred was achieved by culturing excised buds with 2–3 leaflet on a modified MS (1962) basal medium at half-strength supplemented with 0.5, 0.7, 1.0 and 1.2 mg BA/I and 0.1 mg IBA/l. Subculturing the shoots on ½ MS medium supplemented with 0.7 BA and 0.1 mg IBA/l resulted in 9 to 11-fold multiplication every 4 weeks from a single shoot tip or axillary bud, with good shoot extension and leaf growth. BA at 0.7 mg/l was considered optimum because at higher concentrations callus production increased and shoots were dwarfed. Adventitious root formation was induced by subculturing micro-cuttings on the same basal medium for proliferation with 1 mg IBA/l, 0.1 mg BA/l and 2 g AC/l. 90 % rooting was achieved after 2 – 4 weeks. Transfer of the microcuttings to plant growth regulator-free medium also resulted in a 40 % rooting of these cuttings and reduced callus formation. Adaptation of in vitro apple plantlets to greenhouse conditions was achieved by transferring the plantlets in sterile conditions to perlite soaked with ¼ strength MS salts and 0.5 % sucrose for 4 weeks. Then the plantlets were successfully transferred to greenhouse for further acclimatization and later transferred to soil. | |
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