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ISHS Acta Horticulturae 300: In Vitro Culture, XXIII IHC

PROPAGATION OF PYRUS CALLERYANA SEL. D6 BY IN VITRO CULTURE

Authors:   V. ROSSI, G. DE PAOLI, P. DAL POZZO
Abstract:
Pyrus calleryana sel. D6, an important Nashi rootstock, has recently received a fair interest from Italian fruit growers. The possibility to micropropagate it on a commercial scale was investigated. In vitro proliferation rate of shoots was rather high (X 2 – 2,5 every 25 days) on MS medium with 1 mg/1 BAP. Rooting was enhanced by reducing MS macro-elements to half strength, while the addition of phloroglucinol at 8 different doses had no effect. Medium size, compared to short and long microcuttings recorded a maximum of in vitro rooting (90%) and the highest survival (61,5%) during acclimatization in greenhouse.

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