ISHS Acta Horticulturae 297: II International Symposium on Kiwifruit
ISOZYMES FOR CULTIVAR IDENTIFICATION IN KIWIFRUIT |
| Authors: | R. Messina, R. Testolin, M. Morgante |
| Keywords: | polymorphic banding, electrophoresis, phenotypes |
| Abstract:
Fifty-four entries putatively belonging to seven female and two male kiwifruit cultivars were examined for 13 enzyme systems (AAT, ACO, GDH, G6PDH, IDH, MDH, ME, MNR, NDH, 6PGD, PGI, PGM and SKDH). Four enzyme systems, i.e., ACO, MDH, NDH and SKDH, showed identical banding patterns in all clones surveyed. Of the remaining enzymes, AAT, PGI and PGM had the best discriminating power. Six enzyme systems, i.e., GDH, G6PDH, IDH, ME, MNR and 6PGD, though showing polymorphic banding patterns, were poorly resolved. All the New Zealand standard clones were uniquely identified by the simultaneous comparison of the AAT, PGI and PGM zymograms. Some enzyme systems were also polymorphic among plants tentatively ascribed to the same cultivar, thus proving the heterogeneity of kiwifruit material introduced into or propagated in Europe in the early 1970s. | |
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