ISHS Acta Horticulturae 292: Recent Advances in Horticultural Science in the Tropics
EFFECTS OF AUXIN AND CYTOKININ ON IN VITRO ORGANOGENESIS OF CINERARIA CHINENSIS L. |
| Authors: | A.G.Abd. Karim, M. Hamidah |
| Abstract:
Explants from axillary buds of Cineraria chinensis were cultured on solid Murashige and Skoog medium with the addition of 6-benzylaminopurine (BA), kinetin, 2, 4-dichlorophenoxyacetic acid (2, 4-D), 3-indolebutyric acid (IBA), and naphthalene acetic acid (NAA). BA and kinetin tested at a rate of 0 to 20.0 mg/l mostly showed shoot formation, whereas BA at more than 0.1 mg/l produced abnormal shoot formation. An optimal response was obtained using 0.1 mg/l kinetin, and its effects were better than that of BA as the latter caused vitrification of the plantlets. IBA and NAA were used for root induction, where 0.5 mg/l NAA produced the best root formation. 2,4-D instead caused callus formation. Plantlets were successfully transplanted and established in soil after two weeks of conditioning under strong light. Cultures grew vigorously for 75 days. | |
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