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| Authors: | K. Raina, S. Ram |
Abstract:
The investigations on malformin-like substances in malformed mango seedlings were led by corn root curvature bioassay.
The ether soluble fraction of healthy seedlings did not contain any malformin-like activity.
However, the ether soluble fraction of bunchy-top affected tissues resolved into three malformin-like active zones at Rf 0.00–0.20, 0.40–0.50 and 0.70–0.80 on the paper chromatograms developed in solvent system Isopropanol:Ammonia:water (10:1:1 v/v) and they were tentatively designated as VM1, VM2, and VM3. VM2 was more active in malformed tissues as compared to VM1 and VM3. The total malformin activity was high at three periods: February-March, May and July-August.
Dry matter accumulation in malformed tissues fluctuated and was high in February, May and July as compared to a slight fluctuation of dry matter accumulation in healthy tissues.
Malformin concentration increased during dry matter accumulation of malformed tissues.
Therefore, malformins appear to play a role in malformation of mango seedlings.
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