ISHS Acta Horticulturae 289: International Symposium on Plant Biotechnology and its Contribution to Plant Development, Multiplication and Improvement
FURTHER STUDIES ON THE PHOTOSYNTHETIC COMPETENCE AND ACCLIMATIZATION OF ROSA SPECIES EXPLANTS |
| Authors: | S. WALKER, I. SIMPKINS, A.V. ROBERTS |
| Abstract:
Plantlets of Rosa hybrida cvs. Mountbatten and City of Leeds were proliferated on agar based Murashige and Skoog (MS) media containing Sucrose (3 and 4% respectively), BAP (2mg/l), NAA (0.04mg/l, and 0.004mg/l, respectively) and GA3 (0.1mg/l), for 4–6 weeks. Explants were transferred to MS media supplemented with a range of sucrose concentrations (0–5%) and to a simple (sugar-free) hydroponic nutrient medium. Growth rates were recorded and measurements of the carbon dioxide assimilation rates made using an Infra-Red Gas Analyser (IRGA). The use of culture vessels which permitted increased levels of gaseous exchange, together with a reduction in humidity, facilitated the development of photosynthetic competence within explants grown on MS medium supplemented with sucrose and on the simple hydroponic medium. The use of a hydroponic nutrient solution in combination with transplantation plugs for both rooting and acclimatization of explants is also discussed. | |
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