IN VITRO ORGANOGENESIS AND REGENERATION IN CASHEW (ANACARDIUM OCCIDENTALE L.)

Callus formation was obtained from leave and petiole explants when different levels of NAA/BAP or 2-4D/BAP were combined on MS modified medium. Adventitious and secondary roots induction occured only when NAA was present. Induction of embryolds from somatic cells was observed after a 12-month subculture on the adaxial face of cotyledonary explant on SH medium supplemented with NAA/BAP (3/0.8 mg 1^-1). Moreover a morphogenetic gradient along the cotyledons was noted. On these explants, an increase of NAA (6 mg 1^-1) and BAP (1 mg 1^-1) rate induced adventitious root formation after a 3-month subculture.

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