ISHS Acta Horticulturae 289: International Symposium on Plant Biotechnology and its Contribution to Plant Development, Multiplication and Improvement
IN VITRO CONSERVATION OF HAPLOID AND DIPLOID GERMPLASM IN ALLIUM CEPA L. |
| Author: | J. Keller |
| Abstract:
A collection of in vitro clones derived from gynogenetic haploids, diploid regenerants, and their mother plants is being established. Long term maintenance of this material comprises a cycle consisting of a slow growth phase at 4 °C and a multiplication phase using cuttings of in vitro bulblets and cultivation on media with benzylaminopurine (BAP). Maintenance of the haploid constitution is repeatedly checked by stomata guard cell measurements. | |
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