ISHS Acta Horticulturae 289: International Symposium on Plant Biotechnology and its Contribution to Plant Development, Multiplication and Improvement
WIDE HYBRIDIZATION FOR IMPROVEMENT IN CULTIVATED BRASSICAS |
| Authors: | A. Agnihotri, M.S. Lakshmikumaran, V. Jagannathan, K.R. Shivanna |
| Abstract:
The members of genus Brassica are important oilseed crop. B. juncea and B. campestris are the main crucifers grown in India for edible oil. B. napus, mainly grown in Europe, is also grown in northern India. Losses in yield occur due to fungal diseases such as white rust caused by Albugo candida and Alternaria blight caused by Alternaria brassiceae, aphids and drought. B. napus shatters under Indian conditions resulting in heavy losses in yield. Allied species of Brassicas having resistant genes can be used for transfer of resistance into the cultivated Brassicas. We have obtained interspecific hybrids of B. campestris with B. spinescens for transfer of resistance to Alternaria blight. Intergeneric hybrid of B. napus has also been obtained for transfer of shattering resistance from Raphanus. These hybrids have been obtained through sequential culture of ovary, ovule and embryo. The plants showing DNA from both the parents were selected and multiplied through axillary branching and somatic embryogenesis. The sterile F-1 are being maintained in vitro. Attempts are in progress to obtain the colchiploids. The backcross progenies are being screened in the field. | |
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