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ISHS Acta Horticulturae 289: International Symposium on Plant Biotechnology and its Contribution to Plant Development, Multiplication and Improvement

MICROPROPAGATION OF ORNAMENTAL PLANTS

Author:   R.L.M. Pierik
DOI:   10.17660/ActaHortic.1991.289.3
Abstract:
An analysis has been made of commercial micropropagation in 15 West European countries. In 1988 Western Europe had 248 commercial tissue culture laboratories with a total production of 212.5 million plants. Most species micropropagated were ornamental plants (157 million). Special attention has been paid to the Netherlands which has 76 commercial laboratories and had a production of 80 million plants in 1989. A comparison is made of the various micropropagation methods used: single-node culture; axillary branching; adventitious organ formation and callus systems and the chances of obtaining genetic variation and mutations when using these methods is analyzed. The use of inflorescence explants for micropropagation is valuable for a number of ornamental crops, particularly bulbous species such as Hippeastrum, Nerine, and tulip. Ex vitro rooting of in vitro obtained shoots in so-called Rockwool plugs which reduces labour costs is described. One of the most complicated and complex factors influencing micropropagation is the solidifying agent agar, produced from seaweed. A survey is given of agar analysis together with the main effects of using various agars.

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