ISHS Acta Horticulturae 289: International Symposium on Plant Biotechnology and its Contribution to Plant Development, Multiplication and Improvement
IN VITRO REGENERATION OF PLANTLETS OF CANARY ISLAND PINE |
| Authors: | C. Martínez Pulido, I.S. Harry, T.A. Thorpe |
| Abstract:
Adventitious buds of Canary Island pine (Pinus canariensis) were induced on 3-day-old cotyledonary explants cultured on Bornman's MCM medium supplemented with cytokinin. Bud development was enhanced by the elimination of phytohormones, a reduction of mineral salts and sucrose, and the inclusion of activated charcoal (AC) in the medium. Shoots were pulsed in a high concentration of indole butyric acid (IBA) and rooted in peat/vermiculite. | |
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