ISHS Acta Horticulturae 289: International Symposium on Plant Biotechnology and its Contribution to Plant Development, Multiplication and Improvement
IN VITRO REGENERATION OF SHOOT BUDS AND PLANTLET FORMATION FROM MATURE EMBRYOS OF PINUS HALEPENSIS MILL. |
| Authors: | M. Lambardi, K.K. Sharma, T.A. Thorpe |
| Abstract:
Shoot differentiation was obtained from embryonal explants of Pinus halepensis Mill. when cultured on cytokinin-containing media. The concentration and duration of application of N6- benzyladenine (BA), the use of other cytokinins alone or in combination with BA, the basal media, and the age of explants all significantly affected the bud-forming capacity. Shoot elongation was achieved on hormone-free medium and the elongated shoots were rooted by pulsing them with 1 mM indolebutyric acid (IBA) for 6 h. At least 90% of the shoots formed normal roots and the rooted shoots were readily hardened and transplanted to the greenhouse with 100% success. | |
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