ISHS Acta Horticulturae 283: IV International Symposium on Plum and Prune Genetics, Breeding and Pomology
RESPONSE OF SOME MR.S. PLUM CLONES TO IN VITRO PROPAGATION |
| Authors: | S. Morini, F. Loreti, R. Sciutti |
| Abstract:
The clonal selection programme carried out on a population of Myrobalan seedlings has identified some promising clones as rootstocks. The aim of this study was to evaluate the response to micropropagation of some of these clones such as Mr.S. 1/14, Mr.S. 1/3, Mr.S. ¼, Mr.S. 1/6, Mr.S. 1/7, Mr.S. 1/8, Mr.S. 2/3 characterized by a lower vigour compared to Myrobalan B. The culture medium consisted of macro and microelements of MS supplemented with 0.4 mg/l thiamine, 100 mg/l myo-inositol and 30 g/l sucrose. The pH was adjusted to 5.2 before adding 0.6% agar. The following growth regulators were added for the proliferation stage: 0.4 mg/l BAP; 0.08 mg/l IBA; 0.15 mg/l GA3. BAP was eliminated for the elongation stage. Rooting was induced using only 0.5 mg/l IBA. The best proliferation was shown by the Mr.S. ¼ and Mr.S. 1/6, followed by Mr.S. 1/8 and Mr.S. 1/3; the lowest shoot production was given by Mr.S. 1/7, Mr.S. 1/14 and Mr.S. 2/3. Longer shoots were recorded mainly in the cultures of Mr.S. 1/6 and, at lower extent, in those of Mr.S. 1/14 and Mr.S. 1/7. Mr.S. ¼, Mr.S. 1/8 and Mr.S. 2/3 also showed the best morphological aspect among all the clones tested. The rooting stage revealed no statistically significant differences among the clones regarding either the percentage of rooted shoots or the mean number of roots produced. | |
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