ISHS Acta Horticulturae 280: I International Symposium on In Vitro Culture and Horticultural Breeding
USE OF HYPERVIRULENT AGROBACTERIUM TUMEFACIENS CARRYING A BINARY VECTOR TO TRANSFORM EGGPLANT (SOLANUM MELONGENA L.) COTYLEDONARY LEAF DISCS. |
| Authors: | E. Filippone, Paul F. Lurquin |
| Abstract:
Agrobacterium tumefaciens strain A281 carrying plasmid pGA472 was used to stably transform eggplant cotyledonary tissue to kanamycin resistance. Both presence and expression of the neo transgene were demonstrated by DNA dot blot analysis and NPT II enzyme assays. Kanamycin-resistant plantlets were regenerated by organogenesis after six months of culture. | |
Download Adobe Acrobat Reader (free software to read PDF files) | |
