ISHS Acta Horticulturae 280: I International Symposium on In Vitro Culture and Horticultural Breeding
FACTORS INFLUENCING VIRULENCE IN AGROBACTERIUM-MEDIATED TRANSFORMATION OF APPLE |
| Authors: | A.M. Dandekar, S.L. Uratsu, N. Matsuta |
| Abstract:
Relative virulence of Agrobacterium strains may be a key to increased transformation efficiency in apple. We have identified several factors that influence the relative virulence of Agrobacterium strains on leaves of apple. Important among these are (i) the strain of Agrobacterium, (ii) the spatial location of wounds on leaf tissue, (iii) the leaf position on the shoot, (iv) replication regions on introduced binary plasmids and (v) introduction of an extra copy of the transcription activator virG gene on a plasmid stimulates virulence of strain A281 on apple. | |
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