ISHS Acta Horticulturae 280: I International Symposium on In Vitro Culture and Horticultural Breeding
EFFECT OF PHOMA TRACHEIPHILA TOXINS ON CITRUS PROTOPLASTS |
| Authors: | F. Sesto, V. Grimaldi, A.M. Pennisi |
| Abstract:
The action of toxins produced by Phoma tracheiphila, the causal agent of mal secco disease, on citrus protoplasts was studied to investigate if toxin-resistant protoplasts could be used to select mutants resistant to the pathogen. The effect of partially purified toxins on protoplast viability was evaluated by FDA test. A correlation between concentration of toxins and cellular effect was observed. Different sensitivity among protoplasts of 12 species and cultivars of Rutaceae were obtained at the highest toxin concentration. Non-citrus species showed a better tolerance towards toxins. Observation of treated protoplasts by electron microscopy showed ultrastructural changes, similar to those described in "in vivo" infected tissues. | |
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