ISHS Acta Horticulturae 280: I International Symposium on In Vitro Culture and Horticultural Breeding
SELECTIVE ACTION OF ATRAZINE IN CALLUS CULTURES OF SOLANUM NIGRUM AND NICOTIANA TABACUM |
| Authors: | M. De Donato, C. Mozzetti, E. Perucco |
| Abstract:
Callus cultures, obtained from Nicotiana tabacum and from two biotypes (atrazine-resistant and susceptible) of Solanum nigrum, were cultured in the presence of atrazine (0–35–70 μM). Herbicide effects were observed with two sucrose levels (2.5 and 10 g/l) and evaluated by dry weight measurements. The higher sucrose concentration assured a greater growth of the cultures. Atrazine, with both sugar levels, selectively affected the callus proliferation: susceptible cultures bleached within 15 days and were unable to grow, in contrast with the resistant ones, which maintained a green colour and grew similar to the untreated calli. It is possible that growth inhibition depends on secondary effects following the interruption of electron flow at the photosystem II level. | |
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